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ROS levels in these cells were assessed using DCFH-DA staining followed by fluorescence microscopy, revealing that BV2-metformin-loaded PLGA reduced intracellular ROS compared to uncoated nanoparticles and restored mitochondrial membrane potential (MMP) via JC-1 staining

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Senescence-Accelerated Mice Epitalon was also examined in senescence-accelerated mouse strains alongside melatonin

Main Research Findings 1) Treatment with FOXO4-DRI was found to reduce age-related testosterone secretion insufficiency in aged animals by targeting Leydig cells