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Unlike the kinase functions of the PNAK1-3 enzymes the PANK4 enzyme functions to dephosphorylate 4-phosphopantetheine to pantetheine

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Samples (in 40 mM PBS, pH 7.0) were analyzed by capillary electrophoresis (voltage = 30 kV, detection at 195 nm, Agilent 7100, Agilent, Santa Clara, CA, USA) equipped with a polyimidecoated fused silica capillary (68 cm x 50 m) as described previously.22 High-resolution respirometry The respiration of intact and treated cells in the complete medium was measured with the Oroboros Oxygraph-2k (Oroboros Instruments, Innsbruck, Austria)