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While the levels were too low to allow differential S / R quantitation by chiral GC, especially in the presence of the relatively massive levels of S -nicotine, it is considered likely that the S enantiomer was at least dominant because of original formation by reaction of 6-methyl nicotinic acid in the nicotine formation pathway, or subsequent methylation of S -nicotine

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[48] Nicotine is often used compulsively, [49] and dependence can develop within days