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Lysates were sonicated 10 times with 1-s pulses at 80% amplitude on a Fisher Scientific Model 120 Sonic Dismembrator (Thermo Fisher Scientific), then centrifuged at 16,000 g at room temperature (for 9 M urea) or at 4C (for RIPA lysis) for 1520 min

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Comparing treatment options for urea cycle disorders

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