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primarily rough ER
DAPT (GSI-IX) is a potent and orally active γ-secretase inhibitor with IC50s of 115 nM and 200 nM for total amyloid-β (Aβ) and Aβ42
A Dounce homogenizer or a tissue blender is not needed
This extraction method is useful for SDS-PAGE
MinuteTM Plant Cytosolic and Nuclear Protein Isolation Kit 184475-35-2 primarily rough ERThe kit is designed for rapid fractionation of fresh frozen soft plant tissues, especially leaves. Unlike other commercial kit and Lab protocols, of which large amount of starting material (a few grams) and lengthy processing time are required, this kit only uses 100 200 mg starting material. By using a specialized filter cartridge coupled with our proprietary buffer system, four subcellular fractions (cytosol, nuclei, chloroplasts and organelles) can
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