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Rats induced with STZ (60 mg/kg i.v.) and nicotinamide (120 mg/kg i.v.) The diabetic rats were divided into six groups: the diabetic control group and the diabetic treatment group (25) were given compound suspension with a 25 mg/kg BW, respectively

After transfer, membranes were blocked in 1% bovine serum albumin in PBS with 0.1% Tween-20 and probed with anti-PARP (NB100-56599, Novus Biologicals, Littleton, CO), anti-Glutathione (AB19534, Abcam, Cambridge, MA), anti-phospho-IB- (9246), anti-IB- (9242), anti-phospho-IKK- (2594), anti-IKK- (2370), or anti-p65 (8242) (Cell Signaling Technology, Danvers, MA) antibodies

& Walker, C