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Lysates were combined with 6 Laemmli SDS sample buffer (Boston BioProducts, BP-111R) containing 5% 2-mercaptoethanol (VWR Life Science, M131), then loaded onto 420% Criterion TGX pre-cast gels (Bio-Rad, 5671093)

Consistent with our historical practice, we are proposing that if better data become available, we would use those data and the finalized Version 41 of the GROUPER in establishing the FY 2024 MS-LTC-DRG relative weights in the final rule

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