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Briefly, cohorts of 3050 adult flies (approximately 50 mg of fresh tissue) were collected in Eppendorf tubes containing and transferred to 500 ul ice-cold 50 g/l perchloric acid solution containing 0.2 M boric acid and 10 uM gamma-Glu-Glu on ice

The results show that an NO adduct on the sulfhydryl group effectively drives intermolecular disulfide bond formation in TTR, converting native protein into the dsNNTTR species, see Fig