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The ligatures on the CCA, ICA, and PPA were removed, but the ECA was permanently ligated above and below the opening

(2) Loren Pickart, Jessica Michelle Vasquez-Soltero, and Anna Margolina (2015) GHK Peptide as a Natural Modulator of Multiple Cellular Pathways in Skin Regeneration Biomed Research International, 2015, Volume 2015, Page 648108