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Dose selections for SeNPs were conducted according to previous publications with minor modifications ( Semen cryopreservation, drug treatments, and experimental group settings Before semen cryopreservation, six semen samples were individually split into 4 equals and diluted to a final concentration of 6.0 10 7 /ml spermatozoa with the basic extender supplemented with 0 g/ml SeNPs and 0 mM GSH (negative control group, NC), 2 g/ml SeNPs (SeNPs group) ( For semen cryopreservation, semen samples from each group after dilution were loaded onto semen straws and labeled

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In that work, co-immunoprecipitation analysis showed reduced binding between caveolin-1 and eNOS, an interaction normally responsible for limiting eNOS activity