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Additionally, the GLYR1-catalyzed glyoxylate to glycolate conversion in the cytosol and the GOX-catalyzed glycolate to glyoxylate conversion in peroxisome may form a futile cycle that consumes NADPH in hpr1 , whereas the deficiency of GLYR1 in this background saves energy

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Following the indicated treatment, cells on coverslips were gently washed twice with PBS and incubated in 500 L of 4% paraformaldehyde in PBS (Thermo Scientific, J19943-K2) for 10 min