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The day before the assay, Seahorse probe plates were prepared by adding 200 l distilled water, and calibration buffer was added to a 50 ml Falcon tube

a suitable subject or patient in need of therapy are identified by, for example, a health care professional or professionals using known tests, measurements or criteria

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In the oral arm, the vitamin will be administered as: 1 mg/day in weeks 18 and 1 mg/week in weeks 952