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Store lyophilized material at 4 F (20 C) or below Allow vial to reach room temperature before opening Protect from light, heat, and humidity Use sterile laboratory equipment during preparation Avoid repeated freezethaw cycles Label reconstituted samples with preparation date and concentration These practices support consistent experimental outcomes and reduce variability across assays

Single-cell gene expression analysis from a publicly available database [18] suggested dysregulation of both GPX4 as well as iron handling in proximal tubular epithelial cells of the S3 segments, which are known to be the most ferroptosis-prone part of the nephron[24] (Fig

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Mild soreness, redness, or tenderness at the injection site may occur temporarily