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When the cells reached approximately 80% confluency, cells were harvested with trypsin and seeded at a density of 2.9 10 5 cells per well in 2.5 ml of culture medium into 6 well flat-bottomed plates (Costar, Corning, USA) and incubated for 24 hr

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Memorial Sloan Kettering Cancer Center states that NAC interferes with tumor invasion, metastasis, and blood vessel growth in lab experiments, however its not known how well these effects carry over to humans. Clinical studies show there are several ways in which NAC can help defend against certain types of cancer