Exonuclease I, 20,000 U 1111 Exonuclease III is not active

Exonuclease III is not active on 3´-protruding ends of four bases or more in length

depending on the vector

1-kb clone directly from a ligation reaction 2

Alternative toxicity assays

Exonuclease I, 20,000 U 1111 Exonuclease III is not activeDigest ssDNA, but not dsDNA, in a 3 to 5 direction Specific: Processively digest ssDNA in a 3 to 5 direction without harming dsDNA short 3 overhangs in DNA are not digested Flexible: Active under a wide variety of buffer conditions and can be added directly into most reaction mixes Heat Inactivated: Destroy the enzyme activity by incubating at 80C for 15 minutes Exonuclease I digests ssDNA in a 3 to 5 direction,1 3 but does not digest dsDNA. Although