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If I get down to the tiniest amount of light I can see, luminometers can measure 10,000-100,000 times less than that, and the tiniest amount of light I can see is almost too much for my instruments to measure. Our aim as scientists is always to perturb the biological systems we study as little as possible, in order to get as close as we can to natural conditions

The V3 to V4 region of the 16S rRNA gene was amplified via polymerase chain reaction (PCR) employing the 341 F and 805R primers, while the fungal internal transcribed spacer (ITS) region was amplified using the ITS-1F and ITS-1R primers

namely a strong bitter element near the mid-bowl point (more on this to come)

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