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Based on our pre-survey, we assume that the prevalence of tobacco smoking or alcohol drinking among patients with psoriasis is 30% in Shanghai, so we set p=30%, =0.05, =15% of p, and a non-response rate of 10%

Paraffin blocks were sectioned onto SuperFrost Plus slides, dried overnight, and baked for 1 h at 60 C before immediately proceeding with the RNAScope Multiplex Fluorescent v2 assay according to the manufacturers instructions with the following modifications: Target retrieval was performed for 15 min in a boiling beaker, Protease III was used for 30 min pre-treatment at 40 C and hybridized slides were left overnight in 5 SSC before proceeding with the amplification and labeling steps

Meta-analysis on passive smoking at home and lung cancer incidence in men

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