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First, we showed that eryptosis read-out in vitro is dependent on culture conditions

"Efficacy and safety of mildronate for acute ischemic stroke: a randomized, double-blind, active-controlled phase II multicenter trial"

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Datasets with ligands from the XCE analysis were then taken and refined manually using Refmac 57 and Buster 36 to assess ligand density in conventional sigma-A weighted 2mF o -DF c electron density maps