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That is five needle punctures through the rubber stopper, each introducing a tiny risk of contamination

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Additionally, single-molecule fluorescent in-situ hybridization-based microscopic evaluation also indicated that the loss of rze1 increases the level of znf2 transcripts in the nucleus compared to the case of the cytoplasm, suggesting the role of lncRNA in controlling the Y-H morphological transition through the well-characterized morphogenesis regulator, znf2 [100]