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FIGURE 5 Discussion We found here that -Glu-Glu produced excitatory actions on cultured hippocampal neurons by activating partially NMDA receptors, although -Glu-Glu has good affinity for all ionotropic glutamate receptors ( Nevertheless, -Glu-Glu elicited excitatory actions at low concentration (10 M) which were dependent on the integrity of synaptic transmission as TTX (500 nM) blocked both -Glu-Gluelicited [Ca 2+ ]i increases and increase in spontaneous synaptic transmission