An amount of 2 mL of fecal slurry was immediately taken in duplicate: one replicate was immediately frozen at 80 C to have an unstimulated fecal slurry sample for the NGS analysis, whereas the other replicate was acidified with 0.1N HCI (Sigma-Aldrich, 50%/50% v/v), centrifuged at 4 C at 900 x g for 8 min, and filter-sterilized in a 0.22-m cellulose acetate filter
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Alternatively, the HPA axis hypofunction might arise from several mechanisms, including chronic inflammation with elevated TNF- levels, a regulatory T cells response with increased IL-10 and transforming growth factor (TGF)-, heightened oxidative and nitrosative stress pathways, notably elevated nitric oxide (NO) production, and infection-related factors like LPS tolerance and viral infections affecting the HPA axis (see Table 2) [79]
10.1089/ars.2022.0067 33 HauensteinS.ZhangC.-M.HouY.-M.PeronaJ