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( WADA allows athletes to use non-pharmaceutical interventions, such as hypobaric chambers, to raise EPO levels.) Anabolic steroids can cause mood swings, shrunken testicles, baldness, and infertility, and can suppress the bodys ability to produce testosterone on its own

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After the lung extracts were centrifuged (12,000 g , 10 min at 4C), the supernatant was collected, and protein concentrations were determined using a Bicinchoninic Acid (BCA) Protein Assay Kit (Beyotime Institute of Biotechnology)