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Lesson Outline Introduction to Pentose Phosphate Pathway Location: Cytosol of mammary glands, liver, adrenal cortex, adipose tissue, and red blood cells Connection to nucleotide synthesis and NADPH production Oxidative Phase Irreversible reactions Glucose-6-phosphate (G6P) conversion to 6-phosphogluconolactone and eventually to ribulose-5-phosphate G6P dehydrogenase: rate-limiting step Production of first NADPH 6-phosphogluconate dehydrogenase: conversion to ribulose-5-phosphate Production of second NADPH and carbon dioxide Non-oxidative Phase Reversible reactions Phosphopentose isomerase: conversion of ribulose-5-phosphate to ribose-5-phosphate Transketolase: conversion of ribose-5-phosphate to glyceraldehyde-3-phosphate and fructose-6-phosphate Both intermediates enter glycolysis G6PD Deficiency X-linked recessive metabolic disease Hemolytic anemia during periods of oxidative stress Decrease in NADPH and low availability of active glutathione Triggers: infections, inflammatory conditions, fava beans, and certain drugs Peripheral blood smear findings: Heinz bodies and bite cells Hemoglobinuria Prevalence in people of African, Middle Eastern, or Mediterranean descent Protection against malaria Don't stop here

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By blocking NNMT, 5-Amino-1MQ may help restore cellular energy balance and activate SIRT1 pathways associated with metabolic efficiency [3]

The IHC staining with the anti-elastin antibody was performed on the cross sections following Abcam IHC-paraffin protocol