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Mito Stress Test reagents were loaded into the cartridge in sperm respiratory media (Seahorse XF Base Media supplemented with 5.6 mM glucose, 1 mM pyruvate, 2 mL glutamine, 3 mg/mL fat free BSA, pH 6.9), at the concentrations above, except that the final concentration of FCCP was determined to be 1 uM

This system replenishes healthy mitochondria through biogenesis, maintains network integrity via dynamics (fusion/fission), and clears damaged units through mitophagy [148]

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J Pharm Bioallied Sci (2011) 3(3):4667