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Yet another embodiment of the invention, wherein the mainstream cigarette smoke containing very low level of p-BSQ is incapable of producing significant oxidative damage to the lung microsomal proteins of guinea pigs in vivo when the animals are exposed to smoke emitted from the said charcoalfiltered cigarettes in contrast to marked damage of the lung tissue when the animal are exposed to smoke from cigarettes without having the said charcoal filter

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We further quantified the air space size and found that the free distance between gas exchange surfaces was significantly increased in the lungs of ENDS-treated mice, indicating that ENDS caused lung emphysema in ENaC mice ( Figures 1B, C )