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General Procedure C for Coupling the modified Amine 3a to 3i to GDP 6a to 6i The amine ( 3a 3 i , 2.5 mmol, 5 equiv.) was dissolved in dry DMF (5 mL, c = 0.5 M), and slowly added to a solution of GDP 5 ( c = 25 mM in DMFCH 3 OH (134:1), 1 equiv.) and N, N-Diisopropylethylamine (DIPEA, C 8 H 19 N, 129.25 g/mol, 0,76 g/mL, 2.5 mmol, 5 equiv)

Comparison reflects typical conditions in the research-peptide industry, not the terms of any specific supplier

On the evidence question: The absence of Phase III human trial data for compounds like BPC-157 and TB-500 is a genuine clinical limitation, not a technicality

The specificity of miRNA-324-3p was further confirmed by the results demonstrating that overexpression of miRNA-324-3p and transfection with GPX4 mut did not influence the expression of FBI-1 (Figure 2J)