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Materials and methods Cell culture and reagents Cell lines were cultured under the following conditions: PC3, C42B, RM1, PAIII- DMEM (Hyclone SH30243.01), 10% FBS (PEAK PS-FB1), 1% penicillin/streptomycin (P/S) (Gibco 15140-122) and LNCaP- RPMI (Hyclone SH30027.02), 10% FBS, 1% P/S

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Thus, a melting point reflects the thermal energy needed to convert the highly ordered array of molecules in a crystal lattice to the randomness of a liquid