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The Pro-KIV 1.01 software (Applied Photophysics) was used to perform kinetic simulations of the mechanistic models using the determined rate constants from Table 1 based on the indicated reaction schemes

Caspase-1 cleaves the N-terminal structural domain of GSDMD, and the cleaved N-terminal fragment inserts into the cell membrane, to form pores, ultimately causing membrane rupture and the release of pro-inflammatory intracellular contents

-HB exhibits potent anti-inflammatory properties, reducing pro-inflammatory cytokines and microglial activation by inhibiting pathways such as NF-B and NLRP3 inflammasome

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