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Hydroxocobalamin is the form the NHS uses because it lingers in the body longer than cyanocobalamin, and its pharmacology supports dosing intervals of up to three months once levels are stable

Next, membranes were blocked with 5% BSA in Tris-buffered saline containing 0.1% (v/v) Tween 20 (TBST) for 1 h at room temperature and probed with primary antibodies against corresponding antigens overnight at 4 C