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Studies have found distinct bacterial communities in tumor tissues and non-tumor tissues of HCC patients, where bacteria enriched in metastatic nodules, such as Enterococcus and Streptococcus anginosus , can induce an immunosuppressive microenvironment and promote tumor progression through epithelial-mesenchymal transition

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Silanization of coverslips: 18 18 mm glass coverslips (Thermo Fischer Scientific) were placed in a coverslip rack and carefully put into an acetone solution containing 1% (3-aminopropyl) triethoxysilane (APTES) and 1% allyltrimethoxysilane (ATMS) (v/v) and coated overnight 26