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Oxidative stress affects histone acetylation and de-acetylation [69], with indirect perturbation of the epigenetic landscapemethylation tags are transmitted to the offspring

There are pre-clinical reports showing combined anti-tumor effect of pegylated arginine deiminase with other drugs like chloroquine, gemcitabine and TORC1/TORC2 inhibitor P529 in sarcoma, pancreatic cancer and glioblastoma respectively [133,134,135]

The membrane was treated with ECL prime blocking solutions (GE Healthcare, Little Chalfont, UK) and reacted overnight at 4 C with each antibody (Table S1) diluted in blocking buffer