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Discussion Because of the large number of studies that analyzed blood oxidative stress markers in ASD children in recent years, here we were able to include 87 studies with 4928 ASD children and 4181 controls, and revealed that 18 oxidative stress-related markers were dysregulated in blood (serum or plasma) of children with ASD

Blots were incubated overnight at 4 C with primary antibodies and 2 h with HRP-conjugated secondary antibodies at room temperature