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20 of each serial dilution was added to 180 L of blood on ice for final concentrations of 0.0l g/mL, O

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Approximately 500 worms per condition were collected using M9 buffer and pelleted by centrifugation (1000 g , 30 s)

While previous studies have demonstrated that DDAVP can mitigate the risk of overcorrection 21 , some instances have shown it leading to inadequate sodium level increases or even decreases 16,19,20 , especially with unrestricted fluid intake 11