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Interestingly, repeated OS can initiate and maintain elevated levels of IL-1, leading to a positive feedback loop between OS and neuroinflammatory [104]

Animal models, especially gene knockout and transgenic models, have played an indispensable role in mechanistic verification, confirming the causal relationship between key inflammatory mediators (e.g., TNF-) and testicular-penile damage in preclinical settings, and providing a preclinical platform for evaluating the efficacy of potential therapeutic agents (210)

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