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electrochemiluminescence detection of each MSD SULFO-TAG was captured and quantified using SECTOR Imager instrumentation (MSD, Gaithersburg, MD, USA), according to manufacturers protocols as reported previously ( The raw data were analyzed using the Discovery Workbench 4.0 software (MSD) by fitting signal from the control calibration curves to a four-parameter logistic model and then back-fitting the electrochemiluminescence signal from each sample to calculate the unknown concentration

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