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Patch assay on YPD containing the given amounts of H 2 O 2 was carried out with wt W303-1b, W303-1b/gsh1, W303-1b/gsh1/pro1 and W303-1b/gsh1/proB to detect their H 2 O 2 tolerances that normally require GSH

Huang, W

Dissected cochlear epithelia are transferred to permeable membrane inserts (Millicell organotypic culture plate inserts, Millipore) which are placed into 1 mL of culture media (Dulbecco's modified Eagle's medium (high glucose, Glutamax, 25 mM HEPES) with 10% fetal bovine serum, 1% N2 supplement and 10 units/ml penicillin) in a 6-well plate