Holick MF (2007) Vitamin D deficiency
Reversed-phase (RP) HPLC typically uses octadecyl silica (C18) columns in combination with acidic mobile phases, composed of acetonitrile/water [32, 41,42,43], with additives like formic acid (FA) [41, 43, 45, 46], ammonium formate [42, 45, 47, 48], acetic acid (AcA) [49], ammonium acetate [32, 50], trichloroacetic acid (TCA) [38, 51], trifluoroacetic acid (TFA) [32, 50, 52], or ammonium phosphate [53]
Rheumatol Int 34(7):9951004 Acknowledgements PN gratefully acknowledges the support provided by Fundacin La Caixa (LCF/PR/PR13/11080005) and Fundacin Caja Navarra, Gobierno Navarra Salud (12/2017), Fundacin Roviralta, Ubesol, Government of Navarre, Laser Ebro, and Inversores Garcilaso de la Vega S.L
The skin needs a period of complete rest without exposure to potentially irritating ingredients