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Aliquots (3 L) of ethanolic cell pellet extracts were analysed by means of UPLC-TOF MS using the following solvent gradient for chromatography (0.4 mL/min, 45 C): starting with a mixture (10/90, v/v) of water and methanol, the methanol content was increased to 100% within 8 min and, then, kept constant for 1 min

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Yes, it was unpleasant and slightly painful, but it was not as severe as anticipated

Ferroptosis-IN-23 exerts a synergistic effect by simultaneously activating Steap4 and glutathione peroxidase 4 ( GPX4 ), thereby maintaining iron metabolism homeostasis