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Depletion of nicotine-activated neutrophil decreases lung metastasis To examine the functional contribution of nicotine-activated neutrophils to metastatic progression, we performed neutrophil depletion experiment under nicotine-treated or untreated setting by injecting the anti-Ly6G (clone 1A8) monoclonal antibody, which specifically depletes Ly6G + neutrophils but not monocytes 12,65