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Western blot analysis of thoracic aorta proteins showed that the classic aging markers P21 and P16 were significantly downregulated following FOXO4-DRI treatment, whereas the expression levels of the cell proliferation marker Ki-67 and Lamin B, a key protein involved in nuclear structural stability, were markedly upregulated (Figure 1A)

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These results also indicate a change in the expression of synaptic dopamine receptor proteins (D1 and D2), a decreased in serotonergic receptors (5-HT1A and 5-HT2A), metabotropic GABA-B, and muscarinic receptors (18)

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