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The ATP standard curve was generated by replacing the 100-l bacteriaNF mixture with 100 l of tenfold serial dilutions of ATP in M9 medium (from 1 M to 10 pM), following the same assay procedure as used for the bacteriaNF samples

Their short length and defined sequences allow chemists to adjust binding affinity and stability

Since biological activity often correlates with the number of peptide molecules (moles) rather than mass, knowing the molar concentration is essential for accurate dosing in many applications

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