G., Shear, N
Genomic HA-tagged TBC1D9B HeLa Cells were generated with CRISPR/Cas9 mediated knockins using Alt-R sgRNA and ssODN Donors from IDT (Coralville, IA, USA) with the following sequences: Alt-R sgRNA: AGGCATCAGCCGGAAACTCC ssODN Donor: TGATGACACCTTGGGCCAGGCCTCACAGCTGCAGGCATCAGGCGTAGTCGGGGACGTCGTAGGGGTAGCCGGAAACTCCAGGCTGCTCATGGTCACTGGCGGTGCTGAACTGTCTC

Additional POC supplement partners Fullscript (10% off): prevailovercancer.com/fullscript-supplements UltraBotanica (code PREVAIL20): prevailovercancer.com/pathway Flourish Nutraceuticals (code PREVAIL5): flourishrx.com/products/nutrition-assessment-pack The Anticancer Smoothie Frame Whatever protein scoop you select, build the rest of your smoothie around it: Cruciferous sprouts (broccoli, kale) for sulforaphane Berries (low-glycemic, polyphenol-rich) Ground flax or chia for fiber and lignans A green (organic baby spinach, kale) Fat to slow absorption (avocado, MCT, extra virgin olive oil, or a small amount of nut butter) An unsweetened base (filtered water, unsweetened almond, or organic coconut milk) That combination keeps your insulin and IGF-1 spikes blunted, layers in real anticancer phytochemistry, and lets your protein choice serve its job rather than work against you
GGT also identifies liver and brain dysfunction tied to poor diet, alcohol, tobacco, and inactivity