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Specifically, the side chain of R283 6.55 moves towards F279 6.51 and H280 6.52 due to steric effects with the octanoyl chain of ghrelin or the phenyl group of ibutamoren (Fig

After this time, the culture medium was discarded, cells were washed with PBS, and incubated with MTT solution (5 mg/mL) in the dark at 37 C and 5% CO 2 for 4 h