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Using advanced electrophysiological techniques, optogenetic activation, and chemogenetic silencing approaches, studies reveal tesofensine inhibits a specific subset of LH GABAergic neurons, reducing their ability to stimulate food-seeking behavior and consumption

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While in vitro models allow more controlled and complex study designs compared with in vivo models, cultured muscle cell models typically have low endogenous GLUT4 expression [115,116], often lack a mature transverse tubule system [117], and do not recapitulate in vivo insulin responses to the same degree as the 3T3-L1 adipocyte