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4.12 Proteomics and phosphoproteomics data analysis using Perseus Analysis for treatment versus untreated control of peptides and phosphopeptides was performed using Perseus v2.0.9.0

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In addition, we conducted microendoscopic calcium imaging to selectively monitor calcium activity in DMV-projecting PVN neurons by infusing AAV2/Retro-hSyn-Cre virus into the DMV and AAV-DIO-GCaMP6m virus into the PVN of C57 mice, accompanied with the mounting of a GRIN lens at the top of the PVN (Fig

Both endogenous triggers such as ischemia or atherosclerosis and exogenous triggers such as microbial infection can initiate the response