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In cognitive peptides: substitute when possible in vivo tests by cerebral organoids or primary neuronal cultures ( Replacement ), optimise experimental plan to minimise the number of animals ( Reduction ), use appropriate anaesthesia and analgesia during invasive procedures ( Refinement )

This triggers the cAMP signalling cascade, which activates protein kinase A (PKA), leading to hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) activation, resulting in triglyceride hydrolysis in adipocyte cell models

Imagine having a pre-cancerous lesion managed by a biopsy every 3-5 years

The conformational change translates into the formation by the protein of insoluble amyloid aggregates, associated in humans with various neurodegenerative disorders and systemic protein-deposition diseases