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Hence, an effective and secure attachment of a ligand or inhibitor into the tyrosinase catalytic pocket involves interactions with the binuclear copper ions as well as respective coordinated histidine residues and other adjoining residues 92

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Many different conditions affect the contents of intracellular GSH, some of them are the presence of heavy metals, high glucose concentrations, protein malnutrition, thermal shock, exposure to reactive oxygen and nitrogen species including, H 2 O 2 and nitric oxide, ozone exposure, ionizing radiation, and cigarette smoke [37,40,61,73,81]