Methods Cell culture, viruses, and infection Human foreskin fibroblasts (HFF, #86031405, purchased from ECACC) were cultured in DMEM, high glucose, pyruvate (Thermo Fisher #41966052) supplemented with 1 MEM non-essential amino acids (Thermo Fisher #11140050), 1 mM additional sodium pyruvate (Thermo Fisher #11360070), 10% (v/v) fetal bovine serum (FBS, Biochrom #S0115), 200 IU/ml, penicillin (pen), and 200 g/ml streptomycin (strep)
These variants can reduce the bioavailability of Se for SelPs synthesis, compromising antioxidant defense and thyroid hormone regulation [4]
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