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Briefly, a 0.5 ml aliquot of skin homogenate was added to a TCA/HCl solution, vortexed, and incubated at room temperature for 15 min

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Adipose & Metabolic Research Experimental research has explored 5-Amino-1MQ within adipose-related and metabolic research models involving: Adipocyte energy-turnover pathways Lipid-metabolism investigations White-adipose tissue research Energy-expenditure models Metabolic-efficiency pathways Research in this area has contributed to broader scientific interest in obesity-related and metabolic-regulation investigations

Key antibodies used included: anti-FOXO4 (two different clones, ab128908 and sc-373877), anti-StAR, anti-CYP11A1, anti-3-HSD, and anti-Ser15-phospho-p53