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Overview DAY 01: Arequipa Walking City Tour and Santa Catalina Convent Arrival in Arequipa City Tour & Santa Catalina Convent Overnight in a Hotel in Arequipa DAY 02: Arequipa Colca Canyon (Excursion for 2D / 1N Colca Canyon) Colca Canyon Tour Lunch in Chivay Overnight in a Hotel in Chivay DAY 03: Colca Canyon connection Puno Colca Canyon viewpoints Tour Lunch in Chivay Transfer to Puno Overnight in a Hotel in Puno DAY 04: Puno Floating Islands Of Uros Taquile Puno Lake Titicaca Tour (Uros and Taquile Islands) Lunch on the Islands Overnight in a Hotel in Puno DAY 05: Puno Transfer for departure Itinerary Detailed Itinerary: 5-Day Arequipa And Puno Tour Day 1: Welcome to Arequipa City Tour of The White City Reception and exploration of the colonial beauty of Arequipa We begin our adventure with a welcome at the Arequipa airport and a comfortable transfer to the hotel

The membranes were blocked with 5 % skim milk (dilution in TBS with 0.05 % Tween 20 (TBST)) and incubated with primary anti-bodies (1:1000, dilution in TBST) for overnight at 4C